MIL-DTL-87173B(USAF)
APPENDIX C
C.5 PREPARATION OF APPARATUS
C.5.1 Column. Any conventional method of conditioning and installing the column is satisfactory,
provided the system is free of gas leaks and the column resolution meets the minimum requirement
specified in C.5.2.
C.5.2 Column resolution. As a final check on chromatographic equipment and settings, run a sample
of the qualitative calibration mixture and measure the retention times and widths of the n-dodecane and
n-tetradecane peaks as shown on Figure A1. Resolution is defined as the difference in retention times for
n-C12 and n-C14 divided by their average width:
2(RT14 RT12)
R=
W14 + W12
Resolution determined in this way must be at least 5.0 and can be optimized by varying the temperature
program and the carrier flow rate. If resolution cannot be increased to at least 5.0 by optimizing these
parameters, it is necessary to use a different column and follow the general procedure of appendix A.
C.5.3 Chromatograph. Place in service according to the manufacturer's specifications; give particular
attention to flow rates through the detector.
C.6 PROCEDURE
C.6.1 Sample volume shall be approximately 0.5 microliter. This sample is injected neat, which means
no dilution in a solvent.
C.6.2 Inject a measured volume of sample into the chromatograph while simultaneously activating the
temperature program, the integrator, and the recorder.
C.6.3 Integrate the area under the chromatogram continuously until the final peak has been eluted.
(Area under JP-10 peak = AJP-10; Area under MCH peak = AMCH; Area under other peaks = AImpurities in
C.7.1.)
NOTE 3. When calculating area under JP-10, include area under peaks with +10% retention time of
JP-10 if they do not contribute more than 20 percent to the total C-10 peak areas.
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